human growth hormone elisa kit  (Calbiotech)

Journal: Marine Biotechnology (New York, N.y.)

Article Title: Production of Biopharmaceutical Proteins from Scallop Hemocytes as a Bioreactor Using Improved Transfection Conditions

doi: 10.1007/s10126-026-10583-9

Figure Lengend Snippet: Multi-level confirmation of hGH expression in scallop hemocytes. ( A ) Schematic diagrams of the EGFP vector and two hGH vectors tagged with EGFP or V5/His. ( B ) Fluorescence imaging of hGH::EGFP expression in cultured hemocytes (middle). Green and blue signals represent EGFP fluorescence and DAPI-stained nuclei, respectively. The EGFP vector was used as a positive control. ( C ) Comparison of the number of EGFP-positive cells per well expressing either hGH::EGFP protein or EGFP alone ( N = 4). ( D ) Detection of hGH mRNA by semi RT-qPCR (upper) and RT-qPCR (lower) ( N = 3). Labels in X-axis show the expression vector with the presence (+) or absence (–) of the transfection reagent. ( E ) Western blotting for detection of EGFP, hGH::EGFP, and hGH::V5/His proteins using anti-EGFP antibody or anti-hGH antibodies. EGFP produced from E.coli was used as a positive control. ( F ) Western blotting of hGH::V5/His protein under reducing and non-reducing conditions using an anti-V5 antibody. Plus (+) and minus (–) represent treatment of protein samples with a reducing agent or PBS, respectively. ( G ) Quantity of hGH protein from hemocyte lysate measured by ELISA ( N = 5). ( H ) Schematic diagrams of two HiBiT-tagged hEPO expression vectors designed for scallop hemocytes (left) and HEK293 cells (right). ( I ) Relative quantification of hEPO secreted into the culture medium from scallop hemocytes and HEK293 cells using the HiBiT assay. ( J ) Western-blot analysis of hEPO expressed in scallop hemocytes and HEK293, with PNGase F treatment. Plus (+) and minus (–) represent treatment of protein samples with PNGase F or PBS, respectively

Article Snippet: hGH protein was quantified using a Human Growth Hormone ELISA Kit (R & D systems, USA) following the manufacture’s protocol.

Techniques: Expressing, Plasmid Preparation, Fluorescence, Imaging, Cell Culture, Staining, Positive Control, Comparison, Quantitative RT-PCR, Transfection, Western Blot, Produced, Enzyme-linked Immunosorbent Assay, Quantitative Proteomics

Journal: BMC Molecular and Cell Biology

Article Title: Dysregulation of caspase-8 and caspase-9 in T-lymphocyte apoptosis: implications for pathogenesis, diagnosis, and therapeutic targeting in psoriatic arthritis

doi: 10.1186/s12860-026-00565-z

Figure Lengend Snippet: The expression of apoptotic profiles, Bcl-2 and Annexin V in control subjects and Psoriatic arthritic patients (PSA) with different disease activity scores as measured by ELISA. The results showed that bcl-2 as antiapoptotic protein was significantly decreased and Annexin V is significantly increased in PSA patients with different disease activity (mild, moderate, severe) compared to that of the control subjects. However, the change in the apoptotic markers in PSA patients with severe activity significantly higher to that of the corresponding PSA patients with mild and moderate activity as well. Data are presented as mean ± SD of three independent experiments. a p < 0.01, Statistically significant compared to the control subjects. b, c p < 0.001, Statistically significant compared to the results of control, mild, and moderate subjects by using Student’s t-test analysis

Article Snippet: In addition, the assessment of the acute-phase reactant, via the highly sensitive CRP μg/mL (hs-CRP), was carried out using commercially provided ELISA kits (IBL Inc, Cat. No.: IB59126, USA), following the manufacturer’s instructions.Moreover, inflammatory cytokines like tumor necrosis factor- (TNF-α) and interleukin-17(IL-17) were estimated by using both Quantikine Human Immunoassay ELISA kits; (R & D System, Minneapolis, USA) for TNF-α, and Diaclone SAS F-25,020 Besançon Cedex, France kit for determining the level of human IL-17 in serum samples.

Techniques: Expressing, Control, Activity Assay, Enzyme-linked Immunosorbent Assay

Journal: BMC Molecular and Cell Biology

Article Title: Dysregulation of caspase-8 and caspase-9 in T-lymphocyte apoptosis: implications for pathogenesis, diagnosis, and therapeutic targeting in psoriatic arthritis

doi: 10.1186/s12860-026-00565-z

Figure Lengend Snippet: Cellular apoptosis measured by lymphocyte apoptotic index ( A ), and the expression of caspases enzymes (Caspase8, Caspase 9) ( B & C ) in patients of psoriatic arthritis (PsA), as measured by acridine orange-ethidium bromide staining technique and ELISA respectively. Data are presented as mean ± SD of three independent experiments. a p < 0.01, Statistically significant compared to control subjects. b, c p < 0.001, Statistically significant compared to results of control, mild, and moderate subjects by using Student’s t-test analysis. AI: apoptotic index score of T-lymphocyte. PAS: Psoriasis Arthritis

Article Snippet: In addition, the assessment of the acute-phase reactant, via the highly sensitive CRP μg/mL (hs-CRP), was carried out using commercially provided ELISA kits (IBL Inc, Cat. No.: IB59126, USA), following the manufacturer’s instructions.Moreover, inflammatory cytokines like tumor necrosis factor- (TNF-α) and interleukin-17(IL-17) were estimated by using both Quantikine Human Immunoassay ELISA kits; (R & D System, Minneapolis, USA) for TNF-α, and Diaclone SAS F-25,020 Besançon Cedex, France kit for determining the level of human IL-17 in serum samples.

Techniques: Expressing, Staining, Enzyme-linked Immunosorbent Assay, Control